Kleihauer-Betke (KB) Test. The KB test is performed to quantitate the number of fetal cells present in the maternal circulation. Once the size of the feto-maternal. Hemoglobin F quantitation by flow cytometry has been found to be simple, reliable, and more precise than the Kleihauer-Betke test,87 In the CAP. The Kleihauer-Betke test is a somewhat crude laboratory method used to screen maternal blood samples for the presence of fetal red blood cells.

Author: Shaktihn Vudogor
Country: Guatemala
Language: English (Spanish)
Genre: Environment
Published (Last): 23 June 2017
Pages: 383
PDF File Size: 3.19 Mb
ePub File Size: 3.95 Mb
ISBN: 167-9-83573-145-8
Downloads: 57878
Price: Free* [*Free Regsitration Required]
Uploader: Mazugor

To determine if a positive test for FMH indicates the likely cause of fetal death, the percent of total fetal blood volume lost should be calculated, making appropriate adjustments based on the following known relationships:. From Wikipedia, the free encyclopedia.

Kleihauer–Betke test

This rosetting pattern may then be viewed under microscopy. This disruption in the placental barrier may occur for many reasons, including intra-uterine fetal demise and trauma. This is when the Kleihauer-Betke KB test is essential. RBC count Hematocrit Hemoglobin.

In general this is not a problem.

Kleihauer Betke Test – StatPearls. Potential Diagnosis The Rosette test is a qualitative screening test performed on a maternal blood sample to determine if FMH has occurred between a Rh-positive fetus and a Rh-negative mother and serves as a useful screening test.


It is not necessary to draw the sample before induction, onset of labor, delivery, placental delivery etc. Kleihauer-betke testing is important in all cases of maternal trauma. In maternal trauma, the Kleihauer-Betke KB test has traditionally been used testt detect transplacental hemorrhage TPHso that Rh-negative women could receive appropriate Rh immune prophylaxis.

This test has been historically only recommended for the Rh-negative pregnant patient with major trauma. Not to be confused with the Betoe testwhich is also often called a “KB test”. TPH, defined as KB-positive for greater than 0. Please review our privacy policy. Author Information Authors Diann M.

No historical or clinical features reliably identify those in whom it may be the cause of an intrauterine death. Am J Public Health.

During this incubation period, any Rh-positive fetal cells in the maternal sample are sensitized to the anti-Rho D immune globulin and bound. McRoberts maneuver Woods’ betkee maneuver Zavanelli maneuver.

Interfering Factors In the case of maternal persistence of fetal hemoglobin or other maternal hemoglobinopathies that result in elevated HbF, the KB test will be falsely positive and flow cytometry must be used to quantitate the amount of fetal hemorrhage in maternal circulation.



Delivery does result in higher frequency of detection of micro-hemorrhages but this should not confound interpretation of FMH as a possible cause of stillbirth. Nuchal scan Anomaly scan.

Nitro blue tetrazolium chloride test Complete blood count Absolute neutrophil count. There is some controversy on KB testing in the setting of trauma in pregnancy. Calculation of the percentage of fetal vs.

Clear Turn Off Turn On. If positive, it is used to determine additional RhoGAM dosing over the standard or mcg dosage that should be administered. Reasoning that the magnitude of TPH would reflect uterine injury, we evaluated Kleihauer-Betke testing as an independent predictor of preterm labor PTL after maternal trauma. American Journal of Hematology. Trauma lkeihauer the number one cause of pregnancy-associated maternal deaths in the United States Ref: Simple comparative counts allow an estimate of whether a significant fetal-maternal transfusion occurred.

Myeloid blood tests CPT — None of the trauma scoring systems predicted PTL. Some advocate its use in all pregnant trauma patients, including those who are RhD negative.